Nodal
Department : Biotechnology &
Molecular Biology
College of Basic Sciences and Humanities
CCS
Haryana Agricultural University
Hisar –125004
Location : CCS
Haryana Agricultural University
Hisar –125004
Course Coordinator : Prof. and
Head
Department of Biotechnology and Molecular
Biology
SCOPE
Biotechnology is an emerging area
with immense industrial potential. Many
industries have come up with scope of
employment for persons with biology background
and training in Plant and Industrial
Biotechnology. Practical applications of
micropropagation, which is commercially
viable, depends on reducing the production
costs such as that it can compete with seed
production or traditional vegetative
propagation methods e.g. cuttings, tubers,
bulbs and grafting. Biotechniques, mostly
based on the engineering of metabolic
pathways, are now available for modifying many
constituents that are used in the food,
chemical and energy industries. Moreover, the
use of microbes and plants as bioreactors for
the production of foreign, non-plant compounds
is gaining momentum and may eventually lead to
alternative types of agriculture. This may
include production of bioactive pesticides,
vaccines, antibodies and a range of enzymes
which mainly are used in pharmaceutical
industry. The application of molecular
genetics and plant transformation to the
diagnosis and control of plant pests has
become one of the major practical success
stories of plant biotechnology in the past
decade.
FOR WHOM
Postgraduate diploma course is
designed for imparting practical orientation
along with theoretical knowledge. It will be
of one year (Two semesters) duration for
Graduates in Agriculture and General Biology
Stream. And for those who want to enter the
Plant Biotechnology and Industrial
Biotechnology Industries.
EMPLOYMENT
OPPORTUNITIES
The employment generation potential
of Biotechnology sector is much higher than
any other sectors because of many persons
getting employment in the fast emerging
Biotechnology Industry. There is also a
several fold generation of indirect employment
in ancillary and other downstream processing
activities on account of high investment in
Biotechnology Industry.
COURSE DURATION : One year (Two
Semesters)
COURSE CURRICULUM :
|
Course
No. |
Course
Title |
Credit
hrs. |
|
Semester- I |
|
|
|
BMB 451 |
Basics in Biotechnology and Molecular
Biology |
3+0 |
|
BMB 452 |
Applied Molecular Biology |
2+0 |
|
BMB 453 |
Practicals in Molecular Biology |
0+5 |
|
BMB 454 |
Practicals in Plant Tissue Culture |
0+5 |
|
|
|
|
|
Semester- II |
|
|
|
BMB 455 |
Plant Tissue Culture |
2+0 |
|
BMB 456 |
Industrial Biotechnology |
2+0 |
|
BMB 457 |
Practicals in Plant Genetic Transformation |
0+5 |
|
BMB 458 |
Practicals in Industrial Biotechnology |
0+5 |
|
BMB 491 |
Seminar |
1+0 |
Qualifications :
B.Sc (Ag.)/B.Sc.(Medical Stream)
Course Fee : Rs.20,000/-
per Semester (Excluding Boarding & Lodging)
No.
of Participants : Max. 15
Course
Programme for PG Diploma in Biotechnology
BMB
451 Elementary Biotechnology (3+0)
Sem I
Biotechnology background and
importance; Structure, function and
organization of genetic material; Gene
expression and regulation; Gene isolation and
recombinant DNA methods; Gene manipulation;
Biotechnology of microbes; Plant tissue
culture and transgenic crops; Application of
biotechnology for human and animal health;
Biosafety, World trade and Intellectual
property issues.
BMB
452 Applied Molecular
Biology
(2+0) Sem I
History of Molecular Biology; Nucleic
Acids as genetic material; Structure of DNA;
Various forms of RNA; DNA Replication and its
enzymes; Enzymes degrading DNA, Expression,
transcription and translation; Deciphering
genetic code; Protein Synthesis; Post
transcription and post translation
modifications; Organization and regulation of
genes.
BMB
453 Plant Tissue Culture (2+0)
Sem II
History and
scope, Principles underlying in vitro
culture, culture medium and its components,
callus and plant regeneration ,
micro-propagation, synthetic seeds, somaclonal
variation, in vitro production of
haploids, in vitro selection, embryo
culture protoplast culture and somatic cell
hybridization, production of transgenic
plants.
BMB
454 Industrial Biotechnology (3+0)
Sem II
History and scope of Industrial
Biotechnology; Biology of industrially
important organisms; Fermentation, metabolism
and physiology of different organisms;
Genetically modified organisms; enzymes,
secondary metabolites, health care products,
edible vaccines and value added compounds;
Microbes, insects, plants and animals as
bioreactors; Product recovery and processing
methods.
BMB
455 Practicals in Molecular Biology
(0+5) Sem I
Familiarization with laboratory set
up; preparation of solutions, buffers and
media; sterilization and disinfections;
quantification of biomolecules, cells and
growth; extraction, estimation and analysis of
plasmid and genomic DNA and total RNA;
Restriction digestion and electrophoresis
analysis of DNA fragments; Cloning of DNA into
vector and transformation; Southern blotting
and hybridization; Nick translation and probe
labeling; Analysis of proteins by SDS PAGE;
Site specific mutagenesis.
BMB
456 Practicals in Industrial
Biotechnology (0+5) Sem I
Familiarization with
laboratory set up; preparation of media;
fermentor designs; sterilization and
disinfections of reactor; quantification of
biomolecules, cells and growth; fermentation
and estimation of alcohol; analysis of
vinegar, citric acid lactic acid, vitamins,
antibiotics; metabolic manipulation,
production and evaluation of metabolites;
analysis of enzymes – amylases, cellulases and
xylanases; downstream processing and effluent
treatment; value addition of biopesticides and
bioassays.
BMB
457 Practicals in Plant Tissue
Culture (0+5) Sem II
Familiarization with laboratory set
up; Sterilization of media components and
explants; Somatic embryogenesis,
organogenesis, cell suspension cultures, shoot
tip cultures; micro propagation, cell
mutagenesis; anther and microspore culture,
ovary, ovule and embryo culture; protoplasts
isolation, purification and culture.
cryopreservation and plant organ banks.
BMB 458
Practicals in Plant Genetic
Transformation (0+5) Sem II
Plant gene isolation and
cloning; vector construction; introduction of
recombinant plasmid in Agrobacterium;
Agrobacterium mediated and direct gene
transfer methods; transformation of cells and
calli; selection of transformed calli;
regeneration of transgenics; hardening and
transfer to soil; molecular characterization
and confirmation of transgenics.
BMB 491
Seminar
(1+0)