UNDERGRADUATE COURSES
bMb-201
INTRODUCTORY PLANT
BIOTECHNOLOGY
2+0 SEM. II
Scope of plant biotechnology; DNA structure
and function; recombinant DNA techniques; gene
isolation; gene transfer; plant tissue culture;
principles and laboratory protocols;
micropropagation; haploid production; somatic
cell hybridization; transgenic plants;
application of plant biotechnology in
agriculture; biotechnology and society.
POSTGRADUATE
COURSES
BMB-501 PRINCIPLES OF
BIOTECHNOLOGY 3+0 SEM. I
Scope of
biotechnology; introduction of genetic
engineering; plant and animal tissue culture;
fermentation technology; immobilized enzymes;
monoclonal antibodies and hybridoma technology;
embryo transfer technology; application of
biotechnology in agriculture; animal and
veterinary sciences, pharmaceutical industry,
food industry and chemical industry.
BMB-502 MOLECULAR
BIOLOGY-I 3+0
SEM. I
History
of molecular biology; nucleic acids as
hereditary material, structure of DNA;
chromatin, rRNA, tRNA and mRNA; nucleases;
nucleic acid sequencing; DNA replication;
transcription, reverse transcriptase and RNA
replicase; translation; genetic code; operon,
positive and negative control of gene
expression; attenuation; molecular mechanism of
mutation.
BMB-503 MOLECULAR
BIOLOGY-II 2+1
SEM. II
Molecular
organization of cell; structure of genomes;
synthetic chromosomes; replication,
transcription and translation in eukaryotes;
structure of primary transcript; ribozyme, RNA
processing and alternate splicing; molecular
biology of photosynthesis; nitrogen fixation and
stress tolerance; development and
differentiation; molecular evolution.
Practical: Estimation of proteins; RNA and
DNA; SDS-PAGE of proteins; DNA isolation,
purification and characterization;
DNA/RNA/protein blotting; polymerase chain
reaction; DNA restriction analysis; RFLP and
RAPD analysis.
BMB-504/ PRINCIPLES OF
IMMUNOLOGY 2+1 SEM. I
VIM-501 (Basic course
for all Immunology courses)
History
of immunology; antigens, adjuvants, immune
system; organs, tissues and cells;
immunoglobulins, monoclonal antibodies;
recombinant antibodies; theories of generation
of antibody diversity; complement systems,
classical and alternate; cellular interaction in
the immune response; major histocompatibility
complex; cell mediated immune response,
cytokines, immunoregulation; immunological
tolerance, hypersensitivity; mechanisms of
immunity; innate resistance and specific
immunity.
Practical:
Handling, inoculation and
bleeding of laboratory animals; preparation of
antigens and antisera; natural antibodies;
carbon clearance test, lymphoid organs of the
mouse; morphology of the blood leucocytes;
separation of lymphocytes from blood; viable
lymphocyte count, antigen-antibody interaction;
precipitation, immunoelectrophoresis,
agglutination direct and indirect
haemagglutination; complement fixation;
quantitation of immunoglobulins by zinc sulphate
turbidity and single radial immunodiffusion.
BMB-505
genetic
engineering 3+0
SEM.II
Enzymes
used in recombinant DNA research; vectors for
cloning DNA; gene identification; isolation and
development of gene libraries; molecular
cloning; gene mapping and DNA structure
analysis; sequencing and synthesis of double
stranded DNA and complementary DNA; cDNA
libraries; identification and enrichment of
recombinant clones; methods for transfer of
cloned DNA; analysis of expression of
recombinant DNA; site directed DNA alterations
and gene manipulations; cloning in bacteria
other than E. coli; cloning in yeast;
cloning vectors for genes of plant cells;
genetics of tumourogenic DNA region of
agrobacteria and its exploitation; applications
for general improvement of plants; animals and
human beings and their protection against
diseases; biotechnological applications for
production of high value and industrial
products; safety aspects of gene manipulation.
BMB-506 TECHNIQUES IN
BIOTECHNOLOGY 0+3 SEM. I
Isolation
of plasmid and chromosomal DNA from bacteria;
yeast and plants; restriction analysis of
nucleic acids; nick translation; southern
hybridization; in vitro DNA ligation;
cloning in E. coli and cultured plant
cells; nucleic acid sequence determination; site
directed mutagenesis.
BMB-507 PLANT CELL AND TISSUE
CULTURE 2+1 SEM. II
History
and scope, totipotency and cell theory;
principles underlying in vitro culture;
growth and differentiation; culture media and
its constituents; micropropagation; bioreactors;
synthetic seeds; meristem tip culture;
somaclonal variation; anther and microspore
culture; in vitro mutagenesis; production
of secondary metabolites; ovary, ovule and
embryo culture; endosperm culture; protoplast
culture and somatic cell hybridization; gene
transfer methods; transgenic plants;
cryopreservation and germplasm conservation.
Practical: Plant tissue culture research
facilities; preparation of nutrient media;
handling and sterilization of plant material;
isolation, inoculation and subculturing;
establishment of cell suspension cultures;
protoplast isolation and culture; plant
regeneration; demonstration of gene transfer
experiments.
BMB-508/MICRO-505
MICROBIAL BIOTECHNOLOGY 2+1SEM. II
Introduction, scope and
historical development; isolation screening and
genetic improvement of industrially important
microorganisms; type of fermentation systems;
production of various primary and secondary
metabolites, e.g. amino acids, organic acids,
alcohols, enzymes, organic solvents,
antibiotics, etc.; down stream processing; over
production of metabolites; bio-reactor
operations, process control; fermented
beverages; production of single cell protein;
steroid transformation; immobilization of
cells/enzymes; silage production; waste water
treatment; use of genetically engineered
microorganisms in biotechnology; bioinsecticides,
biofertilizers, etc.; food colours and flavours;
retting of flax.
Practical: Isolation of industrially
important microorganisms, their maintenance and
improvement; production of industrial compounds
such as alcohol, beer, citric acid, lactic acid
and their recovery; study of bio-reactors and
their operation; production of biofertilizers.
BMB-509/VMI-513
ANIMAL CELL AND TISSUE CULTURE
1+2 SEM. II
Development of tissue culture technique;
behaviour of cells in terms of
growth,differentiation and metabolism; media for
culturing cells and tissues, natural and defined
media and growth supplement; culture of
different tissues and its applications;
characterization and maintenance of cell lines,
cryo-preservations.
Practical: Nutritional needs of mammalian
cells in tissue culture; short term lymphocyte
culture, fibroblast culture; cell
hybridization; micro manipulation.
BMB-510
BIOTECHNOLOGY-ENVIRONMENT
2+0 SEM. II AND SOCIETY
Environmental impact of industrial effluents;
biosensors; renewable sources of energy; history
of biotechnology regulations; guidelines for
research involving genetically modified
organisms (GMOs); ecological considerations and
recommendations for planned release of GMOs;
biodiversity gene banks; intellectual property
rights (IPR); GATT; TRIPS; farmer's rights;
ethical and socio-economical issues.
BMB-601 ADVANCES IN MOLECULAR
BIOLOGY 3+0 SEM. II
Genome
organization in prokaryotes and eukaryotes;
post-transcriptional and translational events;
DNA-protein interactions; advances in protein
engineering; technological applications of
molecular biology; molecular biology of
specialised tissues, cells and cell organelles;
molecular mechanism of regulation and
differentiation; current topics of molecular
biology.
BMB-602 ADVANCED PLANT GENETIC
3+0 SEM I
ENGINEERING
Plant
genetic engineering and conventional plant
breeding; current techniques of identification
and isolation of plant genes; plant gene
sequences; recent gene transfer strategies for
crop improvement; development of transgenic
plant; expression of transgenes; metabolic
engineering and molecular farming; recent
advances in genetic transformation; ethics and
safety guidelines.
BMB--603 CURRENT TOPICS
IN MICROBIAL BIOTECHNOLOGY
3+0 SEM. I
Fermentative metabolism
and genetic improvement of industrially
important microorganisms through recombinant DNA
technology; current advances in production of
antibiotics, vaccines, biocides and steroid
transformation; bioreactors; bioprocess
engineering; products of non-microbial origin
produced by genetically engineered
microorganisms.
BMB-591
SEMINAR
1+0 SEM. I & II
BMB-600 MASTER'S
RESEARCH
BMB-700 Ph.D. RESEARCH |
